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Proceedings Paper

Photobiomodulation of vascular endothelial and smooth muscle cells in vitro with red laser light
Author(s): Nicholas Kipshidze; Michael H. Keelan; Joseph B. Horn; Victor Nikolaychik
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Paper Abstract

Numerous reports suggest that low power red laser light (LPRLL) is capable of affecting cellular processes in the absence of significant thermal effect. The objective of the present study was to determine the effect of LPRLL on viability, growth, and attachment characteristics of rabbit and human aortic endothelial cells (EC) and smooth muscle cells (SMC) in vitro. All cell cultures were irradiated with single dose LPRLL using a He-Ne continuous wave laser with different energy densities. Assessment of effect on cell viability, growth, and attachment was performed utilizing Alamar Blue assay. Based upon our experiments, we conclude that: 1) stimulation and/or inhibition of cell growth and death can be obtained with LPRLL by varying the energy level, 2) LPRLL increases EC attachment, and 3) EC are more sensitive to photobiomodulation with LPRLL than SMC. These data may have significant importance leading to the establishment of new methods for phototherapy of atherosclerosis and restenosis.

Paper Details

Date Published: 3 December 1996
PDF: 9 pages
Proc. SPIE 2922, Laser Applications in Medicine and Dentistry, (3 December 1996); doi: 10.1117/12.260692
Show Author Affiliations
Nicholas Kipshidze, Medical College of Wisconsin (United States)
Michael H. Keelan, Medical College of Wisconsin (United States)
Joseph B. Horn, Global Therapeutics (United States)
Victor Nikolaychik, Univ. of Wisconsin Medical School (United States)


Published in SPIE Proceedings Vol. 2922:
Laser Applications in Medicine and Dentistry
Gregory B. Altshuler; Fausto Chiesa; Herbert J. Geschwind; Raimund Hibst; Neville Krasner; Frederic Laffitte; Giulio Maira; Reinhard Neumann; Roberto Pini; Hans-Dieter Reidenbach; Andre Roggan; Montserrat Serra I Mila, Editor(s)

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