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Proceedings Paper

Reference and sensing pads for integrated optical immunosensors
Author(s): Juerg Duebendorfer; Rino E. Kunz; E. Mader; Gert L. Duveneck; Markus Ehrat
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Paper Abstract

For integrated optical immunosensors, a much more reliable interpretation of the sensor output signal can be achieved by introducing reference pads located near the sensing pads, in order to separate specific from nonspecific effects. Results of theoretical and experimental investigations are reported for immunosensors based on measuring changes of the effective refractive index due to the binding of analyte molecules. The emphasis is on the correction for temperature variations which is an important example of compensation for nonspecific effects. Using the binding of rabbit immunoglobulin to immobilized protein A as a regenerable model bioaffinity system, the influence of consecutive assay cycles on the two pads has been investigated. Experiments have been performed using sensor chips consisting of TiO2 waveguiding films on fused silica and replicated polycarbonate substrates.

Paper Details

Date Published: 26 November 1996
PDF: 8 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259980
Show Author Affiliations
Juerg Duebendorfer, Paul Scherrer Institute (Switzerland)
Rino E. Kunz, Paul Scherrer Institute (Switzerland)
E. Mader, Ciba-Geigy Ltd. (Switzerland)
Gert L. Duveneck, Ciba-Geigy Ltd. (Switzerland)
Markus Ehrat, Ciba-Geigy Ltd. (Switzerland)

Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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