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Proceedings Paper

Ultrathin oligonucleotide layers for fluorescence-based DNA sensors
Author(s): M. Furch; J. Ueberfeld; Andreas Hartmann; Daniel Bock; Stefan Seeger
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Paper Abstract

Preliminary investigations into the design of an affinity sensor using evanescent wave technology concentrate upon the means of immobilization of the receptor molecules. In this work DNA served as the selective recognition element. The molecular principle of a sequence-selective biosensor for DNA is based on a sandwich-hybridization assay wherein the analyte, a single-stranded (ss)DNA, bound specifically to both an immobilized capture probe and a dye-labeled oligonucleotide in free solution. The efficiency of the capture array depends on the density of highly organized oligonucleotides on the waveguide surface and correlates therefore directly with the specificity and the sensitivity of the sensor. In the present approach using the Langmuir- Blodgett technique cinnamoylbutylether-cellulose monolayers were transferred onto optical fibers or planar waveguides. These films served as matrices for the immobilization of biotinylated oligonucleotides via streptavidin. For the first time streptavidin was immobilized by that manner. The specificity of the streptavidin layer or the following bounded nucleic acid molecules were controlled by an enzyme- linked immunosorbent assay (ELISA). Finally, this application has also shown to be suitable for the detection of Salmonella, which is an important pathogen associated with acute gastroenteritidis and food borne diseases.

Paper Details

Date Published: 26 November 1996
PDF: 7 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259975
Show Author Affiliations
M. Furch, Ruprecht-Karls-Univ. Heidelberg (Germany)
J. Ueberfeld, Ruprecht-Karls-Univ. Heidelberg (Germany)
Andreas Hartmann, Ruprecht-Karls-Univ. Heidelberg (Germany)
Daniel Bock, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Switzerland)


Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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