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Proceedings Paper

Detection of the tumor marker mucine with a diode-laser-based evanescent wave sensor
Author(s): Daniel Bock; Sepp Kaul; Frank Loescher; Thomas Ruckstuhl; V. Schulz; J. Ueberfeld; Stefan Seeger
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Paper Abstract

Optical sensors based on the utilization of the evanescent field arising at the interface between two media in the case of total internal reflection are an excellent tool for the reduction of time consuming and complex chemical analysis. We developed a fiber-optic based set-up with visible diode lasers as excitation sources. As recognition element an optical fiber covered with a photopolymerized antibody monolayer was used. Beside the commercially available cyanine fluorescent dye Cy 5, newly developed fluorescent dyes in the red spectral region were coupled to antibodies. In order to test the set-up in a clinically relevant system the antibodies BM-2 and BM-7 were chosen. With this antibody system the tumor marker mucine in a sandwich immunoassay was investigated. This protein shows increased concentrations in serum and ascites in the case of breast cancer. The combination of semiconductor devices and ultrathin antibody layers together with an antibody system directed against mucine offers the possibility of an on-line detection of the tumor marker.

Paper Details

Date Published: 26 November 1996
PDF: 9 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259973
Show Author Affiliations
Daniel Bock, Ruprecht-Karls-Univ. Heidelberg (Germany)
Sepp Kaul, Univ.-Frauenklinik Heidelberg (Germany)
Frank Loescher, Ruprecht-Karls-Univ. Heidelberg (Germany)
Thomas Ruckstuhl, Ruprecht-Karls-Univ. Heidelberg (Germany)
V. Schulz, Ruprecht-Karls-Univ. Heidelberg (Germany)
J. Ueberfeld, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Germany)


Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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