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Proceedings Paper

Combined optical, electrostatic, and enzymatic handling of single DNA molecules
Author(s): Carsten Hoyer; Shamci Monajembashi; Karl-Otto Greulich
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Paper Abstract

Complete handling of single DNA molecules and their enzymatic restriction are described. For that purpose a microsphere was bound to a DNA molecule and trapped by optical tweezers. It could be moved in any direction. For stretching or rotating the molecule an electrical field was applied while the bead was fixed by the optical tweezers. In a near-equilibrium state of the resulting forces, the DNA remained stretched. Subsequently, a restriction endonuclease was activated by liberating Mg2+ from a caged compound. The enzymatic reaction could be directly observed in the light microscope. While the bead remained in the focus of the laser trap the other restricted part of the DNA molecule is pulled away in the electrical field. The reaction was directly monitored and recorded on videotape.

Paper Details

Date Published: 26 November 1996
PDF: 12 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259970
Show Author Affiliations
Carsten Hoyer, Institute of Molecular Biotechnology (Germany)
Shamci Monajembashi, Institute of Molecular Biotechnology (Germany)
Karl-Otto Greulich, Institute of Molecular Biotechnology (Germany)

Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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