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Proceedings Paper

Image analysis of cell natural fluorescence: diagnostic applications in haematology
Author(s): Monica Monici; Giovanni Agati; Piero Mazzinghi; Franco Fusi; Pietro Antonio Bernabei; Stefano Landini; Pierluigi Rossi Ferrini; Riccardo Pratesi
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Paper Abstract

Haematic cells, excited with radiation of suitable wavelength, give rise to a natural fluorescence (NF) emission. This paper investigates NF to develop new techniques for applications in both basic research and medical diagnostics. Results show that the cell populations examined exhibit peculiar emission bands. The intracellular fluorescence pattern reveals that flouresence is mainly located at cytoplasmic-level, thus related to the metabolic processes of the cells. The photophysical properties of cells appear different among the normal populations and between normal and leukaemic ones. Therefore the recognition of the various cellular elements, according to their fluorescence emission, is possible.

Paper Details

Date Published: 26 November 1996
PDF: 8 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259969
Show Author Affiliations
Monica Monici, Istituto di Elettronica Quantistica/CNR and SDC and CDR Sistemi Cibernetici (Italy)
Giovanni Agati, Istituto di Elettronica Quantistica/CNR (Italy)
Piero Mazzinghi, Istituto di Elettronica Quantistica/CNR (Italy)
Franco Fusi, Univ. degli Studi di Firenze (Italy)
Pietro Antonio Bernabei, Univ. degli Studi di Firenze (Italy)
Stefano Landini, Univ. degli Studi di Firenze (Italy)
Pierluigi Rossi Ferrini, Univ. degli Studi di Firenze (Italy)
Riccardo Pratesi, Area dell Ricerca/CNR and Univ. degli Studi di Firenze (Italy)


Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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