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Proceedings Paper

Indocyanine-green-assisted diode laser optoporation of plant cells
Author(s): A. Pirolo; Marina Mazzoni; U. Vanni; Monica Monici; M. Buiatti; P. Bogani; Riccardo Pratesi
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Paper Abstract

A new technique for the absorption of cells is presented. A NIR diode laser (810 nm) and an absorption enhancing dye (indocyanine green, ICG) are used. Localized heating of ICG aggregates close to the cell surface generates bubbles inside the cells. Expansion and emergence of these bubbles cause the cells to open, thus creating the condition for successful transfection. Successful application to plant (tomato) cells is reported for the first time. The experimental results are directly linked both to the absorption characteristics of the dye employed and to the nature of the wall. Optoporation was achieved only by the use of the dye. Laser powers were employed comparable to those used for animal cells.

Paper Details

Date Published: 26 November 1996
PDF: 4 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259968
Show Author Affiliations
A. Pirolo, Istituto di Elettronica/CNR (Italy)
Marina Mazzoni, Istituto di Elettronica/CNR (Italy)
U. Vanni, Istituto di Elettronica/CNR (Italy)
Monica Monici, CDR Sistemi Cibernetci (Italy)
M. Buiatti, Univ. degli Studi di Firenze (Italy)
P. Bogani, Univ. degli Studi di Firenze (Italy)
Riccardo Pratesi, Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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