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Proceedings Paper

Development of a versatile confocal Raman microscope
Author(s): Robert D. Alcock; Mark Russell Dickinson; Terence A. King
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Paper Abstract

A versatile confocal microscope has been developed that combines the optical sectioning properties of a confocal microscope with the chemical identification characteristics offered by Raman spectroscopy to yield a high performance imaging instrument. The apparatus is described and general alignment procedures discussed for optimum performance. An axial resolution of 1.8 micrometer has been determined experimentally for Raman active planar structure and its form compared to theory, A broad range of applications are envisaged in areas of biology, geology and other areas of microscopic analysis. A study is presented showing its applicability to photodynamic therapy for the localization of specific dyes within cells.

Paper Details

Date Published: 26 November 1996
PDF: 10 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259966
Show Author Affiliations
Robert D. Alcock, Univ. of Manchester (United Kingdom)
Mark Russell Dickinson, Univ. of Manchester (United Kingdom)
Terence A. King, Univ. of Manchester (United Kingdom)

Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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