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Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit
Author(s): Meiai Lin; Shuyu Zhang; Qiao Liu; Changshun Shao; Xuantao Su
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Paper Abstract

The detection of senescent cells becomes increasing important for tumor therapy and drug screening. Here a light sheet microfluidic cytometer with a disposable hydrodynamic focusing unit is developed for two dimensional (2D) light scattering measurements of single cells. The mixed polystyrene microspheres of 3.87 and 2.0 μm in diameter are successfully differentiated by our 2D light scattering microfluidic cytometer. The application of the 2D light scattering microfluidic cytometry for the label-free analysis of senescent cells without any labeling or staining is demonstrated by measurements of H2O2-treated U87 cells. Our light sheet-based 2D light scattering microfluidic cytometer is easy to assemble with a disposable hydrodynamic unit, which may find wild applications in clinics for label-free cell classification.

Paper Details

Date Published: 17 May 2019
PDF: 6 pages
Proc. SPIE 11170, 14th National Conference on Laser Technology and Optoelectronics (LTO 2019), 1117043 (17 May 2019); doi: 10.1117/12.2534145
Show Author Affiliations
Meiai Lin, Shandong Univ. (China)
Shuyu Zhang, Shandong Univ. (China)
Qiao Liu, Shandong Univ. (China)
Changshun Shao, Shandong Univ. (China)
Xuantao Su, Shandong Univ. (China)


Published in SPIE Proceedings Vol. 11170:
14th National Conference on Laser Technology and Optoelectronics (LTO 2019)
Jianqiang Zhu; Weibiao Chen; Zhenxi Zhang; Minlin Zhong; Pu Wang; Jianrong Qiu, Editor(s)

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