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Instantaneous isotropic volumetric imaging of fast biological processes
Author(s): Nils Wagner; Nils Norlin; Jakob Gierten; Gustavo de Medeiros; Bálint Balázs; Joachim Wittbrodt; Lars Hufnagel; Robert Prevedel
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Paper Abstract

Capturing highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light-fields yields 3D images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate Medaka heart and blood flow imaging with single-cell resolution and free of motion artefacts at volume rates <200Hz.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760P (22 July 2019); doi: 10.1117/12.2532242
Show Author Affiliations
Nils Wagner, European Molecular Biology Lab. (Germany)
Nils Norlin, European Molecular Biology Lab. (Germany)
Karolinska Institute (Sweden)
Jakob Gierten, Heidelberg Univ. (Germany)
Univ. Hospital Heidelberg (Germany)
Gustavo de Medeiros, European Molecular Biology Lab. (Germany)
Friedrich Miescher Institute for Biomedical Research (Switzerland)
Bálint Balázs, European Molecular Biology Lab. (Germany)
Joachim Wittbrodt, Heidelberg Univ. (Germany)
Lars Hufnagel, European Molecular Biology Lab. (Germany)
Robert Prevedel, European Molecular Biology Lab (Germany)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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