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Holographic imaging of erythrocytes in acoustofluidic platforms
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Paper Abstract

Acoustofluidics exploits ultrasounds and microfluidic platforms to achieve label-free and contactless manipulation of micro sized objects. Here, we demonstrate the use of off-axis digital holography to investigate the behavior of erythrocytes dispersed in water and exposed to ultrasound standing waves. We consider two different regimes of manipulation. In the first case, the sample is stilled inside the microfluidic channel. Under the influence of acoustic forces, the cells move to the first nodal plane, where they start an aggregation process. We follow the formation of clusters in different regions of the channel, highlighting the different structures that emerge. As a second regime, we monitor the axial position of cells flowing during the application of ultrasuonds. By using a resonance frequency that originates multiple nodal positions, we show how holographic imaging can be used to image the cells distributed in the different nodes.

Paper Details

Date Published: 21 June 2019
PDF: 6 pages
Proc. SPIE 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, 1106014 (21 June 2019); doi: 10.1117/12.2527695
Show Author Affiliations
Teresa Cacace, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello" (Italy)
Univ. degli Studi della Campania “L. Vanvitelli” (Italy)
Pasquale Memmolo, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello" (Italy)
Massimiliano Villone, Univ. degli Studi di Napoli Federico II (Italy)
Marco de Corato, Univ. degli Studi di Napoli Federico II (Italy)
Imperial College London (United Kingdom)
Melania Paturzo, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello" (Italy)
Pier Luca Maffettone, Univ. degli Studi di Napoli Federico II (Italy)
Pietro Ferraro, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello" (Italy)


Published in SPIE Proceedings Vol. 11060:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; Christoph K. Hitzenberger, Editor(s)

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