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Multi-wavelength time-resolved NIRS measurements for estimation of absolute concentration of chromophores: blood phantom study
Author(s): Aleh Sudakou; Frederic Lange; Helene Isler; Anna Gerega; Daniel Ostojic; Piotr Sawosz; Ilias Tachtsidis; Martin Wolf; Adam Liebert
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Paper Abstract

Absolute concentrations of oxyhaemoglobin and deoxyhaemoglobin obtained using multi-wavelength measurements of time-resolved diffuse reflectance signals are presented. The aim was to test how accurately the concentration of oxy- and deoxyhemoglobin can be measured simultaneously at multiple wavelengths. The optical signals were collected using the system constructed by the author’s groupa , which records the distribution in time of flight of photons (DTOFs) simultaneously at 16 spectral channels ranging from 650 to 850 nm. The measurements were carried out on liquid phantoms containing intralipid solution, human blood and yeast in varying amounts. The oxygen saturation of blood covered a continuous range from 100 % to 0 % during 6 deoxygenation cycles. The estimated values of total haemoglobin (20.9, 35.7, 57.3, 45.7 μM) are close to the reference values obtained using a blood gas analyzer (21.3, 37.0, 57.3, 48.0 μM) and the estimated values of absolute concentrations of oxy-, deoxyand total haemoglobin are similar to the values obtained using a commercial frequency-domain NIRS system (OxiplexTS TM ). The phantom measurements have shown the capability to measure the absolute concentrations of chromophores in a studied media using multi-wavelength, time-resolved NIRS technique. The excess number of spectral channels can potentially be used to resolve changes in oxidation state of cytochrome-c-oxidase enzyme.

Paper Details

Date Published: 11 July 2019
PDF: 3 pages
Proc. SPIE 11074, Diffuse Optical Spectroscopy and Imaging VII, 1107422 (11 July 2019); doi: 10.1117/12.2527215
Show Author Affiliations
Aleh Sudakou, Nalecz Institute of Biocybernetics and Biomedical Engineering (Poland)
Frederic Lange, Univ. College London (United Kingdom)
Helene Isler, Univ. Hospital Zürich (Switzerland)
Anna Gerega, Nalecz Institute of Biocybernetics and Biomedical Engineering (Poland)
Daniel Ostojic, Univ. Hospital Zürich (Switzerland)
Piotr Sawosz, Nalecz Institute of Biocybernetics and Biomedical Engineering (Poland)
Ilias Tachtsidis, Univ. College London (United Kingdom)
Martin Wolf, Univ. of Zurich (Switzerland)
Adam Liebert, Nalecz Institute of Biocybernetics and Biomedical Engineering (Poland)


Published in SPIE Proceedings Vol. 11074:
Diffuse Optical Spectroscopy and Imaging VII
Hamid Dehghani; Heidrun Wabnitz, Editor(s)

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