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Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis
Author(s): Anastasiya S. Nerush; Kseniya M. Shсhukina; Irina V. Balalaeva; Anna G. Orlova
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Paper Abstract

Using flow cytometry with a genetically encoded fluorescent sensor, vital dye and two types of apoptosis markers: PE Annexin V and TMRE, the participation of hydrogen peroxide (H2O2) in cisplatin-induced apoptosis was demonstrated. It was shown that cisplatin causes the increase of H2O2 level in early apoptotic cancer cells. Accumulation of H2O2 begins before the externalization of phosphatidylserine but after the loss of mitochondrial membrane potential. Scavenging of H2O2 prevents the cisplatin-induced apoptosis.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 110791M (22 July 2019); doi: 10.1117/12.2527195
Show Author Affiliations
Anastasiya S. Nerush, Institute of Applied Physics (Russian Federation)
Kseniya M. Shсhukina, N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Irina V. Balalaeva, N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Anna G. Orlova, Institute of Applied Physics (Russian Federation)


Published in SPIE Proceedings Vol. 11079:
Medical Laser Applications and Laser-Tissue Interactions IX
Lothar D. Lilge; Carsten M. Philipp, Editor(s)

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