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Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy
Author(s): Björn Kemper; Luisa Pohl; Mathias Kaiser; Eva Döpker; Jürgen Schnekenburger; Steffi Ketelhut
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Paper Abstract

For the example of digital holographic microscopy (DHM), a variant of quantitative phase imaging (QPI), we explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images. The applicability of the proposed numerical evaluation procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. Our results show that histogram-based evaluation of quantitative phase images enables a highly reliable quantitative observation of global cellular morphology changes in confluent cell layers.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760T (22 July 2019); doi: 10.1117/12.2527189
Show Author Affiliations
Björn Kemper, Univ. Münster (Germany)
Luisa Pohl, Univ. Münster (Germany)
Mathias Kaiser, Max-Delbrück-Ctr. für Molekulare Medizin (Germany)
Eva Döpker, Univ. Münster (Germany)
Jürgen Schnekenburger, Univ. Münster (Germany)
Steffi Ketelhut, Univ. Münster (Germany)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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