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Label-free imaging of bipolar cell axons in mouse retina by second-harmonic generation
Author(s): Festa Bucinca-Cupallari; Hyungsik Lim
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Paper Abstract

Here we present label-free imaging of retinal bipolar cell axons by second-harmonic generation microscopy. The nonlinear optical signal arises from uniformly polarized microtubules, providing a surrogate contrast for the functional integrity of axons. The unique properties of microtubule SHG from the inner plexiform layer were employed for testing an outstanding hypothesis in glaucoma, namely, the persistence of BC axons in the disease during a critical period when the synaptic connections to the retinal ganglion cells are lost.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 1107608 (22 July 2019); doi: 10.1117/12.2527161
Show Author Affiliations
Festa Bucinca-Cupallari, Hunter College and The Graduate Ctr. of the City Univ. of New York (United States)
Hyungsik Lim, Hunter College and The Graduate Ctr. of the City Univ. of New York (United States)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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