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Holographic imaging for 3D cells morphology in microfluidic flow
Author(s): Pasquale Memmolo; Massimiliano Villone; Francesco Merola; Martina Mugnano; Lisa Miccio; Pier Luca Maffettone; Pietro Ferraro
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Paper Abstract

The complete cells characterization in microfluidic flow can be achieved by using the quantitative phase imaging by digital holography as imaging tool. In fact, by assuring the complete 3D rotation of flowing cells, it is possible to recover their 3D refractive index mapping by using the tomographic phase-contrast reconstruction. In this paper, we investigate all steps need to obtain the tomographic reconstruction of flowing cells. In particular, we employ a holographic 3D tracking algorithm to follow each cells that moves in the field of view, along with a suitable tracking angle method for the cell’s tumbling. Moreover, a fluid modeling is used to characterize the cell rotation effect. We test the proposed processing pipeline for circulating tumor cells.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110761U (22 July 2019); doi: 10.1117/12.2527129
Show Author Affiliations
Pasquale Memmolo, Institute of Applied Sciences and Intelligent Systems "E. Caianiello" (Italy)
Massimiliano Villone, Univ. of Naples "Federico II" (Italy)
Francesco Merola, Institute of Applied Sciences and Inttelligent Systems "E. Caianiello" (Italy)
Martina Mugnano, Institute of Applied Sciences and Intelligent Systems “E. Caianiello" (Italy)
Lisa Miccio, Institute of Applied Sciences and Intelligent Systems “E. Caianiello" (Italy)
Pier Luca Maffettone, Univ. of Naples "Federico II" (Italy)
Pietro Ferraro, Institute of Applied Sciences and Intelligent Systems “E. Caianiello" (Italy)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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