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In vivo multimodal fibre-probe spectroscopy for glioblastoma detection in mouse model
Author(s): Enrico Baria; Enrico Pracucci; Vinoshene Pillai; Francesco S. Pavone; Gian Michele Ratto; Riccardo Cicchi
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Paper Abstract

Glioblastoma (GBM) is the most common and aggressive malignant brain tumour in adults. Patient survival rates are strongly dependent on the successfully resection of the tumour. In this framework, multimodal optical spectroscopy could provide a fast and label-free tool for improving tumour detection and guiding the removal of diseased tissue. In this study, we used an optical fibre-probe system combining multiple spectroscopic techniques for in vivo examination of normal and GBM tissues in mouse brain. Specifically, the probe – based on a fibre-bundle with optical fibres of various size and properties – allowed performing spectroscopic measurements based on fluorescence, Raman, and diffuse reflectance spectroscopy though two optical windows implanted on the head of each animal. Two visible laser diodes were used for fluorescence spectroscopy, a laser diode emitting in the NIR was used for Raman spectroscopy, and a fibre-coupled halogen lamp for diffuse reflectance. All spectral recordings were done when the animals were anesthetized; optical inspection required less than 4 minutes for each animal. The recorded data were analysed using Principal Component Analysis (PCA) for obtaining an automated classification of the examined tissues based on the intrinsic spectral information provided by Raman and reflectance spectroscopy. The presented method demonstrated high sensitivity and specificity in discriminating GBM from normal brain. Furthermore, we found that the multimodal approach is crucial for improving diagnostic capabilities beyond what can be achieved from individual techniques.

Paper Details

Date Published: 19 July 2019
PDF: 3 pages
Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110731N (19 July 2019); doi: 10.1117/12.2527124
Show Author Affiliations
Enrico Baria, National Institute of Optics (Italy)
Enrico Pracucci, National Enterprise for nanoScience and nanoTechnology (Italy)
Vinoshene Pillai, National Enterprise for nanoScience and nanoTechnology (Italy)
Francesco S. Pavone, National Institute of Optics (Italy)
Univ. of Florence (Italy)
Gian Michele Ratto, National Enterprise for nanoScience and nanoTechnology (Italy)
Scuola Normale Superiore (Italy)
Riccardo Cicchi, National Institute of Optics (Italy)
Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 11073:
Clinical and Preclinical Optical Diagnostics II
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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