Share Email Print

Proceedings Paper • new

Label-free THG imaging of bone tissue microstructure: effect of low gravity on the lacuno-canalicular network
Author(s): Rachel Genthial; Emmanuel Beaurepaire; Marie-Claire Schanne-Klein; Françoise Peyrin; Cécile Olivier; Maude Gerbaix; Laurence Vico; Aurélien Gourrier; Delphine Débarre
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

In bone tissue, multiscale interfaces provide the structural basis of essential bone functions and contribute to its macroscopic mechanical properties. The lacuno-canalicular network (LCN) hosting the osteocytes in the bone matrix, in particular, represents a biological signature of the mechanotransduction activity in response to external biomechanical loading. We have demonstrated that label-free third-harmonic generation (THG) microscopy reveals the structure of the LCN in 3D with submicron precision over millimetric fields of view compatible with histology and can be coupled to second-harmonic generation (SHG) signals relating to the collagen organization in the bone matrix. Taking advantage of these label-free imaging methods, we investigate the impact of microgravity on the LCN structure in mice following a 1- month space flight. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760E (22 July 2019); doi: 10.1117/12.2527029
Show Author Affiliations
Rachel Genthial, Univ. Grenoble Alpes (France)
CNRS (France)
Emmanuel Beaurepaire, Ecole Polytechnique, CNRS, INSERM, Univ. Paris-Saclay (France)
Marie-Claire Schanne-Klein, Ecole Polytechnique, CNRS, INSERM, Univ. Paris-Saclay (France)
Françoise Peyrin, Univ. de Lyon, CNRS, INSERM, Univ. Claude Bernard Lyon 1 (France)
ESRF, European Synchrotron Radiation Facility (France)
Cécile Olivier, Univ. de Lyon, CNRS, INSERM, Univ. Claude Bernard Lyon 1 (France)
ESRF, European Synchrotron Radiation Facility (France)
Maude Gerbaix, Univ. de Lyon (France)
Laurence Vico, Univ. de Lyon (France)
Aurélien Gourrier, Univ. Grenoble Alpes (France)
CNRS (France)
Delphine Débarre, Univ. Grenoble Alpes (France)
CNRS (France)

Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top