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High accuracy platelet counting using lensfree imaging
Author(s): D. Isèbe; E. Gremion; A. Daynès; B. Thouy; N. Rongeat; S. Bressieux; A. Ali-Cherif; V. Rebuffel; P. Joly; S. Morales; O. Cioni; P. Blandin
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Paper Abstract

Flow cytometry is the main technology used in hematology analyzers. However, this technology requires bulky and complex hardware systems. Lens-free imaging is an emerging microscopy technique based on a simple and compact inline holography setup. This technique enables to image a large field-of-view (≈30mm²) leading to statistical counting (>10 000 cells) in a single-shot acquisition consistent with performances required in hematology. We report high accuracy platelet count in 54 platelet-rich plasma samples. This accuracy can be achieved through a wise choice of the illumination spectral properties and an optimized algorithmic chain dedicated to small pure phase objects.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750O (22 July 2019); doi: 10.1117/12.2527008
Show Author Affiliations
D. Isèbe, HORIBA ABX S.A.S. (France)
E. Gremion, HORIBA ABX S.A.S. (France)
A. Daynès, HORIBA ABX S.A.S. (France)
B. Thouy, HORIBA ABX S.A.S. (France)
N. Rongeat, HORIBA ABX S.A.S. (France)
S. Bressieux, HORIBA ABX S.A.S. (France)
A. Ali-Cherif, HORIBA ABX S.A.S. (France)
V. Rebuffel, Univ. Grenoble Alpes (France)
CEA-LETI (France)
P. Joly, Univ. Grenoble Alpes (France)
CEA-LETI (France)
S. Morales, Univ. Grenoble Alpes (France)
CEA-LETI (France)
O. Cioni, Univ. Grenoble Alpes (France)
CEA-LETI (France)
P. Blandin, Univ. Grenoble Alpes (France)
CEA-LETI (France)


Published in SPIE Proceedings Vol. 11075:
Novel Biophotonics Techniques and Applications V
Arjen Amelink; Seemantini K. Nadkarni, Editor(s)

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