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Adaptive fluctuation imaging captures rapid subcellular dynamics
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Paper Abstract

In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, endosomes and nuclei) in living cells using low excitation-light dose. Challenges and opportunities with applying MUSICAL for studying rapid sub-cellular dynamics are discussed.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 110761W (22 July 2019); doi: 10.1117/12.2526846
Show Author Affiliations
Ida S. Opstad, UiT The Arctic Univ. of Norway (Norway)
Florian Ströhl, UiT The Arctic Univ of Norway (Norway)
Åsa B. Birgisdottir, UiT The Arctic Univ. of Norway (Norway)
Sebastián Maldonado, UiT The Arctic Univ. of Norway (Norway)
Trine Kalstad, UiT The Arctic Univ. of Norway (Norway)
Truls Myrmel, UiT The Arctic Univ. of Norway (Norway)
Krishna Agarwal, UiT The Arctic Univ. of Norway (Norway)
Balpreet S. Ahluwalia, UiT The Arctic Univ. of Norway (Norway)

Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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