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X-ray irradiation effects on SH-SY5Y human neuroblastoma cells monitored by means of FTIR micro-spectroscopy
Author(s): Valerio Ricciardi; Marianna Portaccio; Lorenzo Manti; Maria Lepore
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Paper Abstract

Fourier-Transform Infrared micro-spectroscopy (μFT-IR) was used for an in vitro investigation targeted to examine the effects on SH-SY5Y human neuroblastoma cells of X-rays irradiation. μFT-IR thanks to its ability in analyzing cells at a molecular level can be particularly useful in investigating the biochemical changes induced in protein, nucleic acid, lipid, and carbohydrate content of cells after irradiation by graded X-ray doses (0, 2, 4, 6, 8 and 10 Gy). Cell samples were fixed immediately (t0-cells) or 24h after (t24-cells) irradiation. A deconvolution procedure using Lorentzian curves was used for analyzing the collected spectra in order to highlight the changes due to the different treatment. Significant differences were evidenced in spectra from unexposed and exposed cells. Peaks related to important cell components (as Amide and DNA) show significant shifts depending on radiation dose and also the ratios between the intensity of selected bands are affected by the interaction with ionizing radiation. These changes are less evident in t24h-cells, thus suggesting the occurrence of repairing processes of the X-ray induced damage. The results of the present analysis can be helpful in order to develop innovative approaches to monitor radiation cancer radiotherapy outcome so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy.

Paper Details

Date Published: 19 July 2019
PDF: 8 pages
Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110731M (19 July 2019); doi: 10.1117/12.2526399
Show Author Affiliations
Valerio Ricciardi, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Istituto Nazionale di Fisica Nucleare - Sezione di Napolio (Italy)
Marianna Portaccio, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Lorenzo Manti, Istituto Nazionale di Fisica Nucleare - Sezione di Napoli (Italy)
Università degli Studi di Napoli Federico II (Italy)
Maria Lepore, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)


Published in SPIE Proceedings Vol. 11073:
Clinical and Preclinical Optical Diagnostics II
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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