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Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis
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Paper Abstract

Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

Paper Details

Date Published: 19 July 2019
PDF: 3 pages
Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110731W (19 July 2019); doi: 10.1117/12.2526387
Show Author Affiliations
Pavel V. Shalaev, National Research Univ. of Electronic Technology (Russian Federation)
Ekaterina V. Bondina, National Research Univ. of Electronic Technology (Russian Federation)
Sergey A. Dolgushin, National Research Univ. of Electronic Technology (Russian Federation)
Victoria S. Semeykina, Boreskov Institute of Catalysis (Russian Federation)
Novosibirsk State Univ. (Russian Federation)
Natalya N. Sankova, Boreskov Institute of Catalysis (Russian Federation)
Novosibirsk State Univ. (Russian Federation)
Ekaterina V. Parkhomchuk, Boreskov Institute of Catalysis (Russian Federation)
Novosibirsk State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11073:
Clinical and Preclinical Optical Diagnostics II
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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