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Light-induced permeabilization of liposomes for nuclear antibody delivery (Conference Presentation)
Author(s): Ramtin Rahmanzadeh

Paper Abstract

The delivery of therapeutic agents to a specific cellular site is a central challenge in the treatment of different diseases. Liposomes that can release their cargo upon an externally controlled trigger are attractive candidates for localized drug release. Light can facilitate this with high temporal and spatial precision. In this study, we investigated the potential for light-induced release from liposomes with four different photosensitizers. To demonstrate permeabilization of the liposomes, we encapsulated calcein in high concentration inside the liposomes, that the calcein fluorescence is quenched. If calcein is released from the liposome, quenching is avoided and the fluorescence increase can be quantified. We demonstrated that liposomes released the cargo effectively after light irradiation. Most efficient release was observed with liposomes with 5,10-Di-(4-hydroxyphenyl)-15,20-diphenyl-porphyrin added to the lipid film. Further we observed through fractionated irradiation, that most of the calcein release took place during light irradiation, while the permeability of the liposome decreased again after light exposure. Finally, we could show the liposomal light-activated delivery of monoclonal antibodies against the nuclear protein Ki-67 inside living cells. Light irradiation led to a relocalization of the antibodies from endosomal structures to the nucleoli of different cells types.

Paper Details

Date Published: 14 August 2019
Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 110703E (14 August 2019); doi: 10.1117/12.2526071
Show Author Affiliations
Ramtin Rahmanzadeh, Univ. zu Lübeck (Germany)

Published in SPIE Proceedings Vol. 11070:
17th International Photodynamic Association World Congress
Tayyaba Hasan, Editor(s)

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