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Self-sterilizing photoactivated catheters to prevent nosocomial infections
Author(s): F. Amaro; M. Gómez-Mendoza; A. B. Descalzo; L. Rivas; G. Orellana
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Paper Abstract

Bacterial colonization and biofilm formation on catheters are the primary causes of nosocomial infections and entails a limitation for their long-term use, often requiring catheter removal. Ca. 80% of the urinary tract infections contracted by patients in hospitals are catheter-associated, leading to substantial morbidity, mortality and higher costs. The increase of multi-drug resistant bacteria has created an urgent need for new strategies to prevent biofilm formation on catheters and antimicrobial photodynamic inactivation (aPDI) appears to be a promising approach. We have developed (patent pending) self-sterilizing silicone catheters with a covalently attached layer of photosensitizer (PS) that shows strong antibacterial effect in vitro against a panel of bacterial species commonly related to catheter-associated infections such as Pseudomonas aeruginosa and Staphylococcus epidermidis. Illumination with a 532 nm diode laser light of the PSderivatized catheter surfaces induced killing of 99-99.9% of the bacterial biofilm attached to it. The population survival decreased to a negligible level after 60 min of illumination and no significant decrease of the bacterial viability was observed in the absence of either light or PS. Time-resolved luminescence measurements with detection at 1265 nm confirmed generation of singlet molecular oxygen (1O2) only in PS-derivatized catheters upon illumination, paving the way for future applications to reduce the occurrence of catheter-associated urinary tract infections.

Paper Details

Date Published: 7 August 2019
PDF: 6 pages
Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 110708C (7 August 2019); doi: 10.1117/12.2525896
Show Author Affiliations
F. Amaro, Univ. Complutense de Madrid (Spain)
M. Gómez-Mendoza, Univ. Complutense de Madrid (Spain)
A. B. Descalzo, Univ. Complutense de Madrid (Spain)
L. Rivas, Ctr. de Investigaciones Biológicas (Spain)
G. Orellana, Univ. Complutense de Madrid (Spain)


Published in SPIE Proceedings Vol. 11070:
17th International Photodynamic Association World Congress
Tayyaba Hasan, Editor(s)

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