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Label-free, scalable and point-of-care imaging platform for rapid analysis of biomarker
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Paper Abstract

We propose a disruptive point-of-care (PoC) imaging platform based on lens-free interference phase-contrast imaging for rapid detection of biomarker such as for sepsis and potentially other diseases (e.g. cancer). It enables simultaneous analysis of potentially up to 10,000 functionalized microarray spots with different biomarkers with fast time-to-results (few minutes) and by consuming a small sample volume (~10 μL). The high sensitivity allows direct measurements of the biomarker binding without the use of fluorescent labels (e.g. ELISA) or microbial culture methods. In addition, adhoc plasmonic nano-structuring is utilized to significantly improve the sensitivity for biomarker detection (optical path difference ~Å) to concentration levels relevant for disease diagnosis.

The proposed technology incorporates a portable and low-cost lens-free imaging reader made of consumer electronic components, plasmonic microarrays with distinct functionalization, and user-friendly software based on a novel phaseshifting interferometry method for topography and refractive index analysis. Due to its compactness and cost-efficiency, we foresee a great potential for PoC applications, especially for the rapid detection of infectious diseases or lifethreatening conditions, e.g. sepsis, but also for clinical trials of drugs and food control.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 1107619 (22 July 2019); doi: 10.1117/12.2525878
Show Author Affiliations
Luc Duempelmann, ICFO - Institut de Ciències Fotòniques (Spain)
The Barcelona Institute of Science and Technology (Spain)
Roland A. Terborg, ICFO - Institut de Ciències Fotòniques (Spain)
The Barcelona Institute of Science and Technology (Spain)
Josselin Pello, ICFO - Institut de Ciències Fotòniques (Spain)
The Barcelona Institute of Science and Technology (Spain)
Ilaria Mannelli, ICFO - Institut de Ciències Fotòniques (Spain)
The Barcelona Institute of Science and Technology (Spain)
Filiz Yesilkoy, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Alexander A. Belushkin, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Yasaman Jahani, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Nuria Fabri-Faja, Institut Català de Nanociència i Nanotecnologia (ICN2) (Spain)
Priyanka Dey, Institut Català de Nanociència i Nanotecnologia (ICN2) (Spain)
Olalla Calvo-Lozano, Institut Català de Nanociència i Nanotecnologia (ICN2) (Spain)
M.-Carmen Estevez, Institut Català de Nanociència i Nanotecnologia (ICN2) (Spain)
Anna Fàbrega, Hospital Univ. Vall d’Hebron (Spain)
Juan J. González-López, Hospital Univ. Vall d’Hebron (Spain)
Laura M. Lechuga, Institut Català de Nanociència i Nanotecnologia (ICN2) (Spain)
Hatice Altug, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Valerio Pruneri, ICFO - Institut de Ciències Fotòniques (Spain)
The Barcelona Institute of Science and Technology (Spain)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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