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Near-real time monitoring of bacterial viability using the optrode: a portable fluorimeter
Author(s): Fang Ou; Julia Robertson; Cushla McGoverin; Simon Swift; Frédérique Vanholsbeeck
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Paper Abstract

A rapid and easy method for the monitoring of both live and dead bacteria in a sample is valuable in many fields of microbiological and pharmacodynamics studies, and for the monitoring of food safety and public health. Efficient, culture-independent detection of live and dead bacteria can be achieved using differentially staining fluorescent dyes SYTO 9 and propidium iodide (PI). Fluorescence microscopy and flow cytometry have been used extensively for detection of these live/dead cell fluorescence signals, however, both these methods require bulky equipment and are relatively expensive to implement. We are optimising a method to determine live and dead bacterial concentration that takes advantage of an inexpensive fibre-based fluorimeter, the optrode, which can measure fluorescence intensity in bacterial solutions in challenging working environments. The concentrations of live and dead bacteria were predicted using multivariate analysis of optrode-measured fluorescence spectra, which is then compared with results obtained from the flow cytometry measurements. The findings from this study will be used to establish a general method for the monitoring of live and dead bacteria using the optrode.

Paper Details

Date Published: 22 July 2019
PDF: 3 pages
Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 1107916 (22 July 2019); doi: 10.1117/12.2525558
Show Author Affiliations
Fang Ou, The Univ. of Auckland (New Zealand)
The Dodd-Walls Ctr. for Photonic and Quantum Technologies (New Zealand)
Julia Robertson, The Dodd-Walls Ctr. for Photonic and Quantum Technologies (New Zealand)
The Univ. of Auckland (New Zealand)
Cushla McGoverin, The Univ. of Auckland (New Zealand)
The Dodd-Walls Ctr. for Photonic and Quantum Technologies (New Zealand)
Simon Swift, The Univ. of Auckland (New Zealand)
Frédérique Vanholsbeeck, The Univ. of Auckland (New Zealand)
The Dodd-Walls Ctr. for Photonic and Quantum Technologies (New Zealand)


Published in SPIE Proceedings Vol. 11079:
Medical Laser Applications and Laser-Tissue Interactions IX
Lothar D. Lilge; Carsten M. Philipp, Editor(s)

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