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5-ALA/PpIX fluorescent diagnostics of stressed-induced small and large intestine neoplasia in laboratorial rats in vivo
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Paper Abstract

Gastrointestinal tract neoplasia is on the third place of death statistics among oncological diseases, which is associated with the difficulty of early diagnosis of this disease. This study aims to evaluate applicability of delta-aminolevulinic acid / protoporphyrin IX photosensitizer (5-ALA / PpIX) as diagnosing agent for detection of small and large intestine neoplasia. The experiments were performed in vivo on white laboratorial rats. The animals were divided into 2 groups: control and experimental (overpopulation social stress and a diet, including nitrites and amines) ones. The results of the studies, averaged for each type of tissue, clearly show significant differences between normal tissues and malignant intestine tissues of the rats useful for fluorescent-based discrimination of the tissue state. The fluorescence from malignant areas in small and large intestine represented bright red fluorescence with specific maxima at 635 and 704 nm, typical for PpIX, due to the high accumulation of this photosensitizer in the affected tissues. The accumulation of protoporphyrin IX in the intestine cancerous tissues was very well pronounced. A contrast achieved between normal and abnormal tissue sites at 635 nm fluorescence emission was more than an order of magnitude in the case of large intestine neoplasia, which allows using the 5-ALA/PpIX as a sensitive indicator of gastrointestinal carcinoma.

Paper Details

Date Published: 3 June 2019
PDF: 6 pages
Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 1106504 (3 June 2019); doi: 10.1117/12.2523945
Show Author Affiliations
Ekaterina Borisova, Institute of Electronics (Bulgaria)
Saratov State Univ. (Russian Federation)
Alexander Khorovodov, Saratov State Univ. (Russian Federation)
Ilana Agranovich, Saratov State Univ. (Russian Federation)
Aysel Mamedova, Saratov State Univ. (Russian Federation)
Andrey Terskov, Saratov State Univ. (Russian Federation)
Mikita Navolokin M.D., Saratov State Medical Univ. (Russian Federation)
Oxana Semyachkina-Glushkovskaya, Saratov State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11065:
Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine
Elina A. Genina; Valery V. Tuchin, Editor(s)

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