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Amplification of output signal of laser scanning speckle-microscope using gold nanoparticles for detection of Chlamydia trachomatis bacteria. Part 1: theoretical study
Author(s): O. V. Ulianova; N. N. Filonova; I. A. Subbotina; L. A. Dykman; S. S. Ulyanov; S. S. Zaytsev; Yu. V. Saltykov; A. M. Lyapina; I. G. Grashkina; O. S. Larionova; V. A. Feodorova
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Paper Abstract

Method of speckle-microscopy has been adapted to the problem of detection of Chlamydia trachomatis microbial cells in aqueous suspension and fixed on the glass. Combined system “C. trachomatis bacterial cell”-“monoclonal antibody”- “gold nanoparticle” has been used as a complicated scattering element in the case of formation of biospeckles with a small number of scatterers. Optical model of diffraction of strongly focused Gaussian beam on combined scattering system, containing gold nanoparticles, has been suggested. Simulation of speckles, forming with a small number of scattering nanoparticles, has been carried out. It has been demonstrated that the usage of nanoparticles allows to enhance essentially the output signal of speckle-microscope.

Paper Details

Date Published: 3 June 2019
PDF: 9 pages
Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110650O (3 June 2019); doi: 10.1117/12.2523029
Show Author Affiliations
O. V. Ulianova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
N. N. Filonova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
I. A. Subbotina, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
L. A. Dykman, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
S. S. Ulyanov, Saratov State Univ. (Russian Federation)
Federal Research Ctr. for Virology and Microbiology (Russian Federation)
S. S. Zaytsev, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
Yu. V. Saltykov, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
A. M. Lyapina, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
I. G. Grashkina, Saratov State Medical Univ. (Russian Federation)
O. S. Larionova, Saratov State Agrarian Univ. (Russian Federation)
V. A. Feodorova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)


Published in SPIE Proceedings Vol. 11065:
Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine
Elina A. Genina; Valery V. Tuchin, Editor(s)

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