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Detection of blood microcirculation of chicken embryo using LASCA-imaging with application of the new techniques of optical clearing
Author(s): O. V. Ulianova; A. A. Kolosova; N. N. Filonova; A. M. Lyapina; Yu. V. Saltykov; I. A. Subbotina; S. S. Zaytsev; I. G. Grashkina; S. S. Ulyanov; V. A. Feodorova
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Paper Abstract

Methods of t-LASCA and s-LASCA imaging have been adapted to the problem of monitoring of blood microcirculation in naïve chicken embryo. Set-up for LASCA imaging of the model chicken embryo is mounted. The novel original technique of preparing of optical windows for observation of a naïve chicken embryo is suggested. This technique is based on a removal of part of the natural egg shell using a citric acid solution with further optical clearing using glycerol and glucose.

Paper Details

Date Published: 3 June 2019
PDF: 6 pages
Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110650N (3 June 2019); doi: 10.1117/12.2523025
Show Author Affiliations
O. V. Ulianova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
A. A. Kolosova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
N. N. Filonova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
A. M. Lyapina, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
Yu. V. Saltykov, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
I. A. Subbotina, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
S. S. Zaytsev, Federal Research Ctr. for Virology and Microbiology (Russian Federation)
I. G. Grashkina, Saratov State Medical Univ. (Russian Federation)
S. S. Ulyanov, Saratov State Univ. (Russian Federation)
Federal Research Ctr. for Virology and Microbiology (Russian Federation)
V. A. Feodorova, Federal Research Ctr. for Virology and Microbiology (Russian Federation)


Published in SPIE Proceedings Vol. 11065:
Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine
Elina A. Genina; Valery V. Tuchin, Editor(s)

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