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Adsorption kinetics of proteins to biomaterials measured by reflectometric interference spectroscopy
Author(s): Johanna Hutterer; Peter Janoschek; Günter Gauglitz
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Paper Abstract

In this work we present the investigation of the interaction of fibronectin with different biomaterials. Since the amount, type and possible conformational changes of adsorbed proteins at the body-implant interface strongly influences the performance of implants, this work is focusing on a more comprehensive understanding of these processes. To create very stable and thin surface modification of a glass transducer with PEG, PU and PLA an immobilization strategy based on silane chemistry was developed. To monitor the adsorption and desorption of fibronectin onto these biomaterial surfaces, the reflectometric interference spectroscopy – a direct optical method – was applied. To estimate the association and dissociation rate constants, a biomolecular interaction analysis was performed within the kinetically limited regime of the association process. Additionally, the influence of the pH value on the adsorption behavior is exemplary shown for the interaction of fibronectin on a glass surface. These experiments show high potential to give more detailed insights into the complex processes during the implantation of foreign materials into the human body.

Paper Details

Date Published: 11 April 2019
PDF: 7 pages
Proc. SPIE 11028, Optical Sensors 2019, 110282L (11 April 2019); doi: 10.1117/12.2522312
Show Author Affiliations
Johanna Hutterer, Eberhard Karls Univ. Tübingen (Germany)
Peter Janoschek, Eberhard Karls Univ. Tübingen (Germany)
Günter Gauglitz, Eberhard Karls Univ. Tübingen (Germany)


Published in SPIE Proceedings Vol. 11028:
Optical Sensors 2019
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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