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Two-photon laser scanning microscopy integrated with light stimulus system
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Paper Abstract

We demonstrate a home-made two-photon laser scanning microscopy (TPLSM) with a light stimulus system. In this system, the femtosecond pulses are produced by a picosecond fiber laser with pulse width compression. A laser diode serves as stimulus, which modulated by an AOM and coupled into the same light path of a femtosecond laser. The control signal of AOM and trigger signal of two scanning mirrors are synchronized by a FPGA board. With modulating the intensity of CW laser at precise point in one scanning frame, any pattern of light stimulus can be delivered to the sample in real time.

Paper Details

Date Published: 12 March 2019
PDF: 5 pages
Proc. SPIE 11023, Fifth Symposium on Novel Optoelectronic Detection Technology and Application, 1102346 (12 March 2019); doi: 10.1117/12.2521012
Show Author Affiliations
Xin Xu, Suzhou Institute of Biomedical Engineering and Technology (China)
Univ. of Electronic Science and Technology of China (China)
Xingjie Jin, Suzhou Institute of Biomedical Engineering and Technology (China)
Feng Gao, Suzhou Institute of Biomedical Engineering and Technology (China)
Guohua Shi, Suzhou Institute of Biomedical Engineering and Technology (China)


Published in SPIE Proceedings Vol. 11023:
Fifth Symposium on Novel Optoelectronic Detection Technology and Application
Qifeng Yu; Wei Huang; You He, Editor(s)

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