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96-well capped gold nanoslits for backside-reflection plasmonic biosensing
Author(s): Kuang-Li Lee; Po-Cheng Tsai; Meng-Lin You; Pei-Kuen Wei
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Paper Abstract

A nanostructure-based plasmonic biochip with the same size as standard 96-well plates for backside reflection-type biosensing was proposed and validated through analyses of biological interactions. The capped gold nanoslit arrays were fabricated on a polycarbonate plastic film using a rapid hot embossing nanoimprint lithography process. The optical properties of capped gold nanoslits with different structure parameters in backside reflection geometry were studied; their refractive index (bulk) and surface (thickness) sensitivities were verified. By changing the cavity length, the coupling between a broadband cavity resonance and a narrowband surface plasmon resonance mode results in an asymmetric Fano resonance in the reflection spectra. The coupling mode is able to enhance the thickness sensitivity by a factor of 2.4 with wavelength interrogation. The bulk and thickness sensitivities were 454 nm/RIU and 1.14 nm/nm, respectively. The protein-protein interaction experiments verified the sensing capabilities and high sensitivity of the capped nanostructures; a limit of detection (LOD) of 2 ng/mL IgA was achieved. Such a multi-well plate with backside reflection-type geometry, decoupling the optical paths, allows for sensing with opaque, bubbly or highly scattering liquids and benefits multiple sensing applications in the biotechnology and agricultural products.

Paper Details

Date Published: 10 May 2019
PDF: 9 pages
Proc. SPIE 11007, Advanced Environmental, Chemical, and Biological Sensing Technologies XV, 1100704 (10 May 2019); doi: 10.1117/12.2518423
Show Author Affiliations
Kuang-Li Lee, Research Ctr. for Applied Sciences - Academia Sinica (Taiwan)
Po-Cheng Tsai, Research Ctr. for Applied Sciences - Academia Sinica (Taiwan)
Meng-Lin You, Research Ctr. for Applied Sciences - Academia Sinica (Taiwan)
Pei-Kuen Wei, Research Ctr. for Applied Sciences - Academia Sinica (Taiwan)


Published in SPIE Proceedings Vol. 11007:
Advanced Environmental, Chemical, and Biological Sensing Technologies XV
Tuan Vo-Dinh, Editor(s)

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