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Surface-enhanced Raman spectroscopy of chloroalkanes in microfluidic chips
Author(s): Zdeněk Pilát; Martin Kizovský; Jan Ježek; Stanislav Krátký; Jaroslav Sobota; Martin Šiler ; Ota Samek ; Tomáš Buryška ; Pavel Vaňáček ; Jiří Damborský ; Zbyněk Prokop ; Pavel Zemánek
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Paper Abstract

Optofluidics, a research discipline combining optics and microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples, e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we have developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

Paper Details

Date Published: 18 December 2018
PDF: 7 pages
Proc. SPIE 10976, 21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 1097619 (18 December 2018); doi: 10.1117/12.2518393
Show Author Affiliations
Zdeněk Pilát, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Martin Kizovský, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Stanislav Krátký, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Jaroslav Sobota, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Martin Šiler , Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Ota Samek , Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Tomáš Buryška , Masaryk Univ. (Czech Republic)
Pavel Vaňáček , Masaryk Univ. (Czech Republic)
Jiří Damborský , Masaryk Univ. (Czech Republic)
Zbyněk Prokop , Masaryk Univ. (Czech Republic)
Pavel Zemánek , Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 10976:
21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Pavel Zemánek, Editor(s)

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