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Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips
Author(s): Jan Ježek; Zdeněk Pilát; Silvie Bernatová; Johanna Kirchhoff; Astrid Tannert; Ute Neugebauer; Ota Samek; Pavel Zemánek
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Paper Abstract

Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Because of the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We used laser tweezers Raman spectroscopy (LTRS) in a microchamber chip to manipulate and analyze individual E. coli cells. We subjected the cells to antibiotic cefotaxime, and we observed the changes by the time-lapse microscopy and Raman spectroscopy. We found observable changes in the cellular morphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments [18].

Paper Details

Date Published: 18 December 2018
PDF: 8 pages
Proc. SPIE 10976, 21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 1097618 (18 December 2018); doi: 10.1117/12.2517933
Show Author Affiliations
Jan Ježek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Zdeněk Pilát, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Silvie Bernatová, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Johanna Kirchhoff, Universitätsklinikum Jena (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Leibniz Institute of Photonic Technology (Germany)
Astrid Tannert, Universitätsklinikum Jena (Germany)
Leibniz Institute of Photonic Technology (Germany)
Ute Neugebauer, Universitätsklinikum Jena (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Leibniz Institute of Photonic Technology (Germany)
Ota Samek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 10976:
21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Pavel Zemánek, Editor(s)

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