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Time-Lapse imaging of bactericidal effect on nanostructural surface
Author(s): Takeshi Ito; Kazuki Nakade; Keisuke Jindai; Takashi Sagawa; Hiroaki Kojima; Tomohiro Shimizu; Shoso Shingubara
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Paper Abstract

We observed the changes in fluorescence intensity which was based on fluorescent protein (mCherry) when the fluorescent protein expressed E-coli attached on the natural and artificial nanostructural surface. Time-lapse imaging was used to analyze the changes in fluorescence intensity caused by the effusion of intercellular fluid, including fluorescent protein, at the single cell level. We found that there were three stages of changes in the fluorescence intensity gradient. These characteristics were observed both on the natural and artificial nanostructural surface.

Paper Details

Date Published: 13 March 2019
PDF: 6 pages
Proc. SPIE 10965, Bioinspiration, Biomimetics, and Bioreplication IX, 109650N (13 March 2019); doi: 10.1117/12.2514492
Show Author Affiliations
Takeshi Ito, Kansai Univ. (Japan)
Kazuki Nakade, Kansai Univ. (Japan)
Keisuke Jindai, Kansai Univ. (Japan)
Takashi Sagawa, National Institute of Information and Communications Technology (Japan)
Hiroaki Kojima, National Institute of Information and Communications Technology (Japan)
Tomohiro Shimizu, Kansai Univ. (Japan)
Shoso Shingubara, Kansai Univ. (Japan)


Published in SPIE Proceedings Vol. 10965:
Bioinspiration, Biomimetics, and Bioreplication IX
Raúl J. Martín-Palma; Mato Knez; Akhlesh Lakhtakia, Editor(s)

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