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Label-free and fluorescence photonic crystal biochips for early cancer biomarker detection
Author(s): A. Sinibaldi; N. Danz; E. Sepe; P. Munzert; A. Occhicone; M. Allegretti; E. Giordani; P. Giacomini; F. Michelotti
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Paper Abstract

We report on the development of a biosensing platform that combines label-free and fluorescence based detection on disposable Bloch surface wave biochips. This system is applied to the detection of the HER2-neu/ErbB2 clinical biomarker related to breast cancer development. We first describe the design and fabrication of the BSW biochips as well as the principle of operation of the optical reading instrument. Then, the approaches for surface functionalization and immobilization of proteins for specific detection on the biochips are discussed. Finally, experimental results on a sandwich immunoassay for ErbB2 detection in cell lysates are presented.

Paper Details

Date Published: 7 March 2019
PDF: 7 pages
Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 1089507 (7 March 2019); doi: 10.1117/12.2514310
Show Author Affiliations
A. Sinibaldi, Sapienza Univ. of Rome (Italy)
N. Danz, Fraunhofer Institute for Applied Optics and Precision Engineering (Germany)
E. Sepe, Sapienza Univ. of Rome (Italy)
P. Munzert, Fraunhofer Institute for Applied Optics and Precision Engineering (Germany)
A. Occhicone, Sapienza Univ. of Rome (Italy)
M. Allegretti, Regina Elena National Cancer Institute (Italy)
E. Giordani, Regina Elena National Cancer Institute (Italy)
P. Giacomini, Regina Elena National Cancer Institute (Italy)
F. Michelotti, Sapienza Univ. of Roma (Italy)


Published in SPIE Proceedings Vol. 10895:
Frontiers in Biological Detection: From Nanosensors to Systems XI
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

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