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Zebrafish xenograft breast cancer models for high-throughput drug response screening
Author(s): Rebecca Schmitz; Alex J. Walsh; Kelsey Tweed; Steve Trier; Anna Huttenlocher; Melissa C. Skala
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Paper Abstract

The heterogeneity and dynamic nature of cancerous tumors, such as those seen in breast cancer, pose a unique challenge in determining treatment regimens. The use of zebrafish as an in vivo model of breast cancer provides a high-throughput model with the potential to screen for efficacious drugs on a patient-by-patient basis. In this study, we use two-photon microscopy to measure metabolic changes in zebrafish with xenografted breast cancer tumors before, during, and after treatment with the anti-cancer drug paclitaxel. We use this metabolic imaging data to evaluate the zebrafish as a robust in vivo model of breast cancer. Preliminary results suggest the xenograft tumors respond to treatment with paclitaxel at 48 hours post treatment, as demonstrated by significant changes in NAD(P)H fluorescence lifetimes.

Paper Details

Date Published: 4 March 2019
PDF: 7 pages
Proc. SPIE 10888, Biophysics, Biology and Biophotonics IV: the Crossroads, 1088804 (4 March 2019); doi: 10.1117/12.2513688
Show Author Affiliations
Rebecca Schmitz, Univ. of Wisconsin-Madison (United States)
Morgridge Institute for Research, Univ. of Wisconsin-Madison (United States)
Alex J. Walsh, Univ. of Wisconsin-Madison (United States)
Morgridge Institute for Research, Univ. of Wisconsin-Madison (United States)
Kelsey Tweed, Univ. of Wisconsin-Madison (United States)
Morgridge Institute for Research, Univ. of Wisconsin-Madison (United States)
Steve Trier, Univ. of Wisconsin-Madison (United States)
Morgridge Institute for Research, Univ. of Wisconsin-Madison (United States)
Anna Huttenlocher, Univ. of Wisconsin-Madison (United States)
Melissa C. Skala, Univ. of Wisconsin-Madison (United States)
Morgridge Institute for Research, Univ. of Wisconsin-Madison (United States)


Published in SPIE Proceedings Vol. 10888:
Biophysics, Biology and Biophotonics IV: the Crossroads
Adam Wax; Vadim Backman, Editor(s)

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