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Correlated simultaneous fluorescence and phosphorescence lifetime imaging for metabolic mapping and oxygen sensing in living cells
Author(s): Sviatlana Kalinina; Patrick Schaefer; Bjorn von Einem; Angelika Rück
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Paper Abstract

In combination with laser scanning microscopes, optical imaging technologies based on time correlated single photon counting (TCSPC) are successfully used in fluorescence lifetime imaging microscopy (FLIM) providing monitoring of intracellular intrinsic metabolic coenzymes as NAD(P)H (nicotinamide adenine dinucleotide (phosphate)). Due to oxygen-dependent quenching of the phosphorescence of some compounds including transition metal complexes, the phosphorescence lifetime imaging microscopy (PLIM) can be used for evaluation of oxygen partial pressure (pO2). Using a multi-channel FLIM/PLIM system, we were able to monitor pO2 by PLIM simultaneously with NAD(P)H by FLIM providing complex metabolic and redox imaging of living cells and tissues.

Paper Details

Date Published: 22 February 2019
PDF: 1 pages
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088232 (22 February 2019); doi: 10.1117/12.2513590
Show Author Affiliations
Sviatlana Kalinina, Univ. Ulm (Germany)
Patrick Schaefer, Ctr. for Mitochondrial and Epigenomic Medicine, The Children's Hospital of Philadelphia (United States)
Bjorn von Einem, Univ. Ulm (Germany)
Angelika Rück, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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