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Machine learning for segmenting cells in corneal endothelium images
Author(s): Chaitanya Kolluru; Beth A. Benetz; Naomi Joseph; Harry J. Menegay; Jonathan H. Lass; David Wilson
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Paper Abstract

Images of the endothelial cell layer of the cornea can be used to evaluate corneal health. Quantitative biomarkers extracted from these images such as cell density, coefficient of variation of cell area, and cell hexagonality are commonly used to evaluate the status of the endothelium. Currently, fully-automated endothelial image analysis systems in use often give inaccurate results, while semi-automated methods, requiring trained image analysis readers to identify cells manually, are both challenging and time-consuming. We are investigating two deep learning methods to automatically segment cells in such images. We compare the performance of two deep neural networks, namely U-Net and SegNet. To train and test the classifiers, a dataset of 130 images was collected, with expert reader annotated cell borders in each image. We applied standard training and testing techniques to evaluate pixel-wise segmentation performance, and report corresponding metrics such as the Dice and Jaccard coefficients. Visual evaluation of results showed that most pixel-wise errors in the U-Net were rather non-consequential. Results from the U-Net approach are being applied to create endothelial cell segmentations and quantify important morphological measurements for evaluating cornea health.

Paper Details

Date Published: 13 March 2019
PDF: 10 pages
Proc. SPIE 10950, Medical Imaging 2019: Computer-Aided Diagnosis, 109504G (13 March 2019); doi: 10.1117/12.2513580
Show Author Affiliations
Chaitanya Kolluru, Case Western Reserve Univ. (United States)
Beth A. Benetz, Case Western Reserve Univ., Univ. Hospitals Eye Institute (United States)
Cornea Image Analysis Reading Ctr. (United States)
Naomi Joseph, Case Western Reserve Univ. (United States)
Harry J. Menegay, Case Western Reserve Univ., Univ. Hospitals Eye Institute (United States)
Cornea Image Analysis Reading Ctr. (United States)
Jonathan H. Lass, Case Western Reserve Univ., Univ. Hospitals Eye Institute (United States)
Cornea Image Analysis Reading Ctr. (United States)
David Wilson, Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 10950:
Medical Imaging 2019: Computer-Aided Diagnosis
Kensaku Mori; Horst K. Hahn, Editor(s)

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