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Studying nucleic membrane fluctuations of tumor cells treated with chemotherapeutic drug using confocal reflectance quantitative phase microscopy (Conference Presentation)

Paper Abstract

Confocal reflectance quantitative phase microscopy system is developed in our lab to quantify nuclear membrane fluctuations. This system able to provide the µm level depth resolved phase information of the back scattered signal from nucleic membrane at ms temporal resolution. The phase information quantify the height fluctuations of nucleic membranes, which are subject to thermal fluctuations around the stable equilibrium in viscoelastic mediums. We further combined this system with Brillouin spectroscopic system, which measures longitudinal modulus of the nuclear material in the gigahertz (GHz) frequency range. Combining the information from confocal phase and Brillouin spectroscopy provides the nuclear membrane and material mechanical properties. Studies of anti-cancer drug effect on nuclear stiffness is performed on human lung cancer cells. Chemotherapeutic agent Doxorubicin (Dox) were used to treat these cancer cells and mechanical properties of nucleus were studied using the combined confocal and Brillouin spectroscopic system, as discussed above. The combined study of membrane fluctuations and stiffness measurements represent the positive correlation and indicate the softening the nuclei of tumor cells after treating with chemotherapeutic drug.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10887, Quantitative Phase Imaging V, 108871G (4 March 2019); doi: 10.1117/12.2513575
Show Author Affiliations
Vijay Raj Singh, Massachusetts Institute of Technology (United States)
Zahid Yaqoob, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 10887:
Quantitative Phase Imaging V
Gabriel Popescu; YongKeun Park, Editor(s)

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