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Wide-field quantitative phase imaging and diffraction tomography with Fourier ptychography (Conference Presentation)
Author(s): Chao Zuo; Jiaji Li
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Paper Abstract

High-throughput quantitative phase imaging (QPI) is essential to cellular phenotypes characterization as it allows high-content cell analysis and avoids adverse effects of staining reagents on cellular viability and cell signaling. Among different approaches, Fourier ptychographic microscopy (FPM) is probably the most promising technique to realize high-throughput QPI by synthesizing a wide-field, high-resolution complex image from multiple angle-variably illuminated, low-resolution images. However, the large dataset requirement in conventional FPM significantly limits its imaging speed, resulting in low temporal throughput. In this talk, we report two optimum illumination schemes for FPM to achieve high-speed or even single-shot QPI. We present the high-speed imaging results of in vitro Hela cells mitosis and apoptosis at a frame rate of 25 Hz with a full-pitch resolution of 655 nm at a wavelength of 525 nm (effective NA = 0.8) across a wide field-of-view (FOV) of 1.77 mm2, corresponding to a space–bandwidth–time product of 411 megapixels per second. We also discuss how FPM can be extended to optical diffraction tomography (ODT) under Born or Rytov approximation, achieving super and depth resolved 3D imaging over a wide FOV.

Paper Details

Date Published: 4 March 2019
PDF
Proc. SPIE 10887, Quantitative Phase Imaging V, 1088709 (4 March 2019); doi: 10.1117/12.2513257
Show Author Affiliations
Chao Zuo, Nanjing Univ. of Science and Technology (China)
Jiaji Li, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10887:
Quantitative Phase Imaging V
Gabriel Popescu; YongKeun Park, Editor(s)

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