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Application of plasmonic nanoprobes for SERS sensing and imaging of biotargets in plant systems
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Paper Abstract

The knowledge over gene expression dynamics and location in plants is crucial for applications ranging from basic biological research to agricultural biotechnology (e.g., biofuel development). However, current methods cannot provide in vivo dynamic detection of genomic targets in plants. This limitation is due to the complex sample preparation needed by current methods for nucleic acids detection, which disrupt spatial and temporal resolution. We report the development of a unique multimodal method based on plasmonics-active nanoprobes, referred to inverse molecular sensitnels capable of in vivo imaging and biosensing of microRNA biotargets within whole plant using surface-enhanced Raman scattering (SERS) detection. This work lays the foundations for in vivo functional imaging of RNA biotargets in plants with previously unmet spatial and temporal resolution for many applications ranging from agricultural biotechnology to biofuel research.

Paper Details

Date Published: 7 March 2019
PDF: 6 pages
Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108940C (7 March 2019); doi: 10.1117/12.2512010
Show Author Affiliations
Pietro Strobbia, Duke Univ. (United States)
Bridget M. Crawford, Duke Univ. (United States)
Hsin-Neng Wang, Duke Univ. (United States)
Rodolfo Zentella, Duke Univ. (United States)
Maxim I. Boyanov, Institute of Chemical Engineering (Bulgaria)
Argonne National Lab. (United States)
Zhen-Ming Pei, Duke Univ. (United States)
Tai-Ping Sun, Duke Univ. (United States)
Kenneth M. Kemner, Argonne National Lab. (United States)
Tuan Vo-Dinh, Duke Univ. (United States)


Published in SPIE Proceedings Vol. 10894:
Plasmonics in Biology and Medicine XVI
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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