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Gold nanoprisms enhanced optical coherence tomography angiography of live animals in the second near infrared window (Conference Presentation)
Author(s): Peng Si; Edwin Yuan; Orly Liba; Yonatan Winetraub; Siavash Yousefi; Elliott SoRelle; Derek Yecies; Rebecca Dutta; Adam de la Zerda
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Paper Abstract

Optical coherence tomography angiography (OCTA) is an important tool for investigating vascular networks and microcirculation in living tissue. Traditional OCTA detects blood vessels via intravascular dynamic scattering signals derived from the movements of red blood cells (RBCs). However, the low hematocrit and long latency between RBCs in capillaries make these OCTA signals discontinuous, leading to incomplete mapping of the vascular networks. OCTA imaging of microvascular circulation is particularly challenging in tumors due to the abnormally slow blood flow in angiogenic tumor vessels and strong attenuation of light by tumor tissue. Here we demonstrate in vivo that gold nanoprisms (GNPRs) can be used as OCT contrast agents working in the second near infrared window, significantly enhancing the dynamic scattering signals in microvessels and improving the sensitivity of OCTA in skin tissue and melanoma tumors in live mice. This is the first demonstration that nanoparticle-based OCT contrast agent works in vivo in the second near infrared window, which allows deeper imaging depth by OCT. With GNPRs as contrast agents, the post-injection OCT angiograms showed 41% and 59% more microvasculature than pre-injection angiograms in healthy mouse skin and melanoma tumors, respectively. By enabling better characterization of microvascular circulation in vivo, GNPR-enhanced OCTA could lead to better understanding of vascular functions during pathological conditions, more accurate measurements of therapeutic response, and improved patient prognoses.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10867, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII, 108671K (4 March 2019); doi: 10.1117/12.2511528
Show Author Affiliations
Peng Si, Stanford Univ. (United States)
Edwin Yuan, Stanford Univ. (United States)
Orly Liba, Stanford Univ. (United States)
Yonatan Winetraub, Stanford Univ. (United States)
Siavash Yousefi, Stanford Univ. (United States)
Elliott SoRelle, Stanford Univ. (United States)
Derek Yecies, Stanford Univ. (United States)
Rebecca Dutta, Stanford Univ. (United States)
Adam de la Zerda, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 10867:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII
James G. Fujimoto; Joseph A. Izatt, Editor(s)

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