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Brain imaging with extended-focus optical coherence tomography at different scales and spectral ranges (Conference Presentation)
Author(s): Paul J. Marchand; Arno Bouwens; Daniel Szlag; Theo Lasser; Jérôme Extermann
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Paper Abstract

Over the past decades, optical coherence tomography has emerged as an important imaging technique to study biological processes through its ability to perform three-dimensional imaging at high acquisition rates and non-invasively. Furthermore, OCT has shown a growing interest in brain imaging through its capacity in obtaining functional information such as cellular viability, hematocrit and blood flow velocity. Although OCT can reach image depths spanning a few millimeters, the effective imaging depth is typically dictated by the depth-of-field of the imaging optics. In traditional OCT systems, this depth-of-field is given by the Rayleigh range and is thus coupled to the lateral resolution. As such, increasing the numerical aperture of the system reduces the imaging depth, ultimately hampering the depth-multiplexing advantage of OCT. Wavefront engineering schemes have been devised to overcome this limitation, providing the OCT systems with an extended-focus. We present here two extended-focus OCT systems (xf-OCT) optimized for cerebral imaging. The first system operates in the visible wavelength range and is designed to image the superficial cortex of mice at high contrast and at high resolution. Its high axial and lateral resolution of 0.8 and 1.4 um respectively, maintained over 200 um enable resolving structures such as myelinated axons, neuronal cells and micro-vessels in vivo. The second system is optimized for deep microvascular cortical imaging and operates in the infrared spectral range. Through its extended-focus and increased penetration, the second system can provide maps of cortical microvasculature over 800 um in depth in the cortex in vivo.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10867, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII, 108671U (4 March 2019); doi: 10.1117/12.2511462
Show Author Affiliations
Paul J. Marchand, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Arno Bouwens, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Daniel Szlag, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Jérôme Extermann, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 10867:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII
James G. Fujimoto; Joseph A. Izatt, Editor(s)

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