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Analog mean-delay method: a new time-domain super-resolution technique for accurate fluorescence lifetime measurement
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Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.

Paper Details

Date Published: 22 February 2019
PDF: 5 pages
Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840D (22 February 2019); doi: 10.1117/12.2510937
Show Author Affiliations
Dug Young Kim, Yonsei Univ. (Korea, Republic of)
Wonsang Hwang, Yonsei Univ. (Kosovo, Republic of)
Dong Eun Kim, Yonsei Univ. (Korea, Republic of)
Youngjae Won, Osong Medical Innovation Foundation (Kosovo, Republic of)
Sucbei Moon, Kookmin Univ. (Korea, Republic of)
Sang Yoon Lee, INTEKPLUS Co., Ltd. (Korea, Republic of)
Min Gu Kang, INTEKPLUS Co., Ltd. (Korea, Republic of)
Won Sub Han, INTEKPLUS Co., Ltd. (Korea, Republic of)

Published in SPIE Proceedings Vol. 10884:
Single Molecule Spectroscopy and Superresolution Imaging XII
Zygmunt Karol Gryczynski; Ingo Gregor; Felix Koberling, Editor(s)

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