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Acoustofluidic platform for in-channel immunoassays
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Paper Abstract

We demonstrate proof of concept for a point-of-care diagnostic that is used for the detection of chloride channel accessory 1 (CLCA1), a key regulator of mucus production. The prototypical device utilizes ultrasound-confined polystyrene (PS) microspheres held in a longitudinal standing bulk acoustic wave (LSBAW) as reaction substrates. Pressure field amplification between two included pillar arrays enables immobilization of these antigen-coated beads in a predetermined low-pressure region perpendicular to the direction of flow. Bronchoalveolar lavage (BAL) samples from IL-13 stimulated pigs were incubated overnight in a solution of untreated PS beads prior to channel introduction. A PZT- 8 piezoceramic transducer was used to actuate the channel (f1,E = 575 kHz) to focus and confine the beads in a linear zero-pressure node. A solution of two proprietary anti-CLCA1 monoclonal antibodies (mAbs) modified with sulfocyanine3 (Cy3) NHS ester were flowed (7 μL/min, 15 min) into the channel and incubated for 1.5 hours. A solution of phosphate-buffered saline was then used to remove excess antibodies prior to channel/bead cluster imaging. The bead solution was collected, under no acoustic actuation, at the outlet for analysis by flow cytometry. Increased fluorescence over control samples (p<0.0001) demonstrated that the LSBAW platform can serve as a functional immunoassay to allow for serial, contactless reagent washes and fluorescent probe introduction.

Paper Details

Date Published: 7 March 2019
PDF: 7 pages
Proc. SPIE 10893, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI, 1089304 (7 March 2019); doi: 10.1117/12.2510887
Show Author Affiliations
Michael M. Binkley, Washington Univ. in St. Louis (United States)
Mingyang Cui, Washington Univ. in St. Louis (United States)
Jennifer Yantis, Washington Univ. School of Medicine in St. Louis (United States)
Shamus P. Keeler, Washington Univ. School of Medicine in St. Louis (United States)
Benjamin J. Gerovac, Washington Univ. School of Medicine in St. Louis (United States)
Derek E. Byers, Washington Univ. School of Medicine in St. Louis (United States)
Michael J. Holtzman, Washington Univ. School of Medicine in St. Louis (United States)
J. Mark Meacham, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 10893:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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