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Exploratory investigation of the spatial relationships of collagen and nerves in subcutaneous white adipose tissue (scWAT) using 2-photon microscopy
Author(s): Patrick W. Breeding; Magdalena Blaszkiewicz; Kristy Townsend; Andre Khalil; Karissa B. Tilbury
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Paper Abstract

In obesity, subcutaneous white adipose tissue (scWAT) is often marked by reduced adipogenesis, hypoxia, adipocyte hypertrophy, and impaired lipolysis, all of which contribute to overall metabolic dysfunction. The role of the peripheral nervous system is appreciated in the control of WAT lipolysis; sympathetic denervation in WAT blocks lipolysis to a variety of lipolytic stimuli. Yet, we believe additional processes and work is needed to more fully understand adipose hypertrophy. Obese adipose tissue is fibrotic with excess deposition of collagen, yet there is limited work demonstrating the impact of fibrosis on overall tissues structure and peripheral nerves of the scWAT. Here we present a multiphoton approach to image both the peripheral nerves labeled with Alexa 488 and collagen via Second Harmonic Generation (SHG) imaging to investigate the impact of obesity and related fibrosis. The degree of colocalization between the nerve and collagen tissues was examined using both the Pearson’s coefficient and a recently adapted astrophysics technique, the Metric Space Technique (MST). In preliminary findings, both colocalization approaches demonstrated an increase colocalization of nerve and collagen in the aged and obese mice. The MST technique has multiple output functions that are used to examine morphological features such as structure of the nerve/collagen network as well as the circularity of these structures. Both colocalization techniques showed different in the obese mice and indicated a more diffuse circular nerve/collagen network. Collectively, these metrics provide important quantification of the nerve/collagen interaction and the role of the peripheral nervous function in maintaining appropriate adipose function.

Paper Details

Date Published: 22 February 2019
PDF: 8 pages
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088218 (22 February 2019); doi: 10.1117/12.2510753
Show Author Affiliations
Patrick W. Breeding, Univ. of Maine Chemical and Biomedical Engineering (United States)
Magdalena Blaszkiewicz, Univ. of Maine School of Biology and Ecology (United States)
Univ. of Maine Graduate School of Biomedical Science and Engineering (United States)
Kristy Townsend, Univ. of Maine School of Biology and Ecology (United States)
Univ. of Maine Graduate School of Biomedical Science and Engineering (United States)
Andre Khalil, Univ. of Maine Chemical and Biomedical Engineering (United States)
Univ. of Maine Graduate School of Biomedical Science and Engineering (United States)
CompuMAINE Lab. (United States)
Karissa B. Tilbury, Univ. of Maine Chemical and Biomedical Engineering (United States)
Univ. of Maine Graduate School of Biomedical Science and Engineering (United States)


Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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