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Binary wavefront optimization for multi-photon intravital microscopy through intact cranial bone (Conference Presentation)

Paper Abstract

Scattering is a major obstacle on the way of imaging deeper than a few mean-free-paths through bone. The high density of mineralization and collagen fibers deposition make bone a very inhomogeneous tissue that produces severe scattering. Although long wavelength excitation extends the mean-free-path for multi-photon microscopy, however imaging more than 150 microns through bone suffers from loss of resolution and intensity. We previously simulated the wavefront distortions caused by bone using phase accumulation ray tracing (PART) method. Our findings show that some low-order optical aberrations can be corrected using traditional adaptive optics systems such as a deformable mirror, however, a significant amount of high order aberrations are remaining, which require a secondary correction method to restore the point spread function at depth. In this work, we use a high-speed binary wavefront correction method using a digital light processor (DLP) to correct the wavefront in a hostile environment such as bone. We use the PART method to produce an initial estimate of the wavefront, and use a genetic algorithm to evolve it to an optimum using maximum intensity metric. The binary wavefront correction produces a factor of 21 enhancement and the initialization using PART method increases the enhancement 2.5 times.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10886, Adaptive Optics and Wavefront Control for Biological Systems V, 1088605 (4 March 2019); doi: 10.1117/12.2510726
Show Author Affiliations
Kayvan Forouhesh Tehrani, The Univ. of Georgia (United States)
Peter Kner, The Univ. of Georgia (United States)
Luke J. Mortensen, The Univ. of Georgia (United States)

Published in SPIE Proceedings Vol. 10886:
Adaptive Optics and Wavefront Control for Biological Systems V
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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