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Leukocyte dynamics in cutaneous acute graft-versus-host disease by noninvasive laser confocal video microscopy: a cross-sectional pilot study (Conference Presentation)
Author(s): Inga Saknite; Michael Byrne; Madan Jagasia; Eric R. Tkaczyk
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Paper Abstract

Inflammatory tissue response is one of the first and most common manifestations of acute graft-versus-host disease (aGVHD), a potentially deadly immune-mediated disease that occurs in 30-60% of patients after stem cell transplantation. A fundamental challenge in developing effective treatment strategies for aGVHD is the lack of tools to study disease biology in real-time in post-transplant patients. The inflammatory tissue response causes increased expression of specialized endothelial proteins on vessel walls making leukocytes to roll, adhere and eventually extravasate into the tissue at a higher rate than in normal conditions. Although the importance of leukocyte-endothelial interactions to detect and track inflammation has been well shown in murine models, there are no published clinical studies in humans. In this study, we explore the feasibility to detect presence of aGVHD in post-transplant patients through the imaging of in vivo leukocyte motion. We used a clinical confocal microscope (Vivascope 1500) to acquire videos of 5 aGVHD patients and 5 controls (no aGVHD) within 50±30 days post-transplant. The microscope is capable of real-time imaging of individual cells in the postcapillary vessels at 9 frames per second. Through video analysis, we extracted five quantitative parameters: number and velocity of rolling leukocytes, number of adherent leukocytes (stationary >30 s), blood flow velocity, and number of vessels. In a limited number of subjects, we show that parameters characteristic of the dynamic motion in skin capillaries can be observed noninvasively in post-transplant patients. Further studies are needed to test the diagnostic potential of these parameters.

Paper Details

Date Published: 13 March 2019
Proc. SPIE 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics, 108850D (13 March 2019); doi: 10.1117/12.2510585
Show Author Affiliations
Inga Saknite, Vanderbilt Univ. Medical Ctr. (United States)
Michael Byrne, Vanderbilt Univ. Medical Ctr. (United States)
Madan Jagasia, Vanderbilt Univ. Medical Ctr. (United States)
Vanderbilt-Ingram Cancer Ctr. (United States)
Eric R. Tkaczyk, Vanderbilt Univ. Medical Ctr. (United States)
Vanderbilt Univ. (United States)
Tennessee Valley Healthcare System (United States)

Published in SPIE Proceedings Vol. 10885:
Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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