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Intracellular dynamics with quantitative phase imaging (Conference Presentation)
Author(s): Sherazade Aknoun; Anaïs Saintoyant; Antoine Federici; Benoit Wattellier
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Paper Abstract

It is now known that interaction between cells and their environment or between intracellular compartments is based on complex vesicular transport processes [1]. This transport consists in material internalization from the external environment and compartment exchanges inside the cell itself. Understanding the mechanisms and regulation of this intracellular trafficking is an intense object of study in the field of cellular biology. The goal is to understand how vesicles transporting proteins and lipids are targeted to specific cellular compartments and fused with the membrane. Progress about intracellular trafficking is currently essentially made by constant innovation in fluorescence based techniques. They now reach single molecule resolution in living cells. It is possible to follow molecules all along their travel inside the cell. In this case, the main limitation is fluorescent probes could bias the vesicle behaviors and alter their transport inside the cell. Quantitative phase imaging techniques are conventionally used in microscopy, enhancing the contrast for imaging semi-transparent samples with a non-invasive (i.e. label free) and fast approach. For instance, phase correlation imaging introduced by [2] showed that global statistics on the whole cell or a population can give insight of motions and dynamics. We propose to study in details the behavior of vesicles at the intracellular scale and their interaction with the cytoskeleton. The resolution and the high sensitivity brought by a High Definition wave front sensor allows following all individual vesicles through time follow up, while imaging the whole cell. We can study their interaction with the surrounding environment (Brownian or guided motion along the cytoskeleton). We will show how we can extract information from quantitative phase images about the intracellular transport and the influence of the intracellular order/disorder. [1] Tokarev AA, Alfonso A, Segev N. Overview of Intracellular Compartments and Trafficking Pathways. In: Madame Curie Bioscience Database [Internet]. Austin (TX): Landes Bioscience; 2000-2013. [2] Phase correlation imaging of unlabeled cell dynamics, Lihong Ma and al., Scientific Reports volume 6, Article number: 32702 (2016)

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10887, Quantitative Phase Imaging V, 108871K (4 March 2019); doi: 10.1117/12.2510536
Show Author Affiliations
Sherazade Aknoun, PHASICS S.A. (France)
Anaïs Saintoyant, PHASICS S.A. (France)
Antoine Federici, PHASICS S.A. (France)
Benoit Wattellier, PHASICS S.A. (France)

Published in SPIE Proceedings Vol. 10887:
Quantitative Phase Imaging V
Gabriel Popescu; YongKeun Park, Editor(s)

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